关键词:

"> 麝香酮, 人脐静脉内皮细胞, 氧化应激, 细胞凋亡

Abstract:

This study aims to investigate the protective effect and mechanism of muscone on human umbilical vein endothelial cells (HUVECs) induced by H₂O₂. The impacts on HUVECs cell proliferation vitality were evaluated through MTS assay;The morphology of HUVECs was observed by eosin staining method;The intracellular reactive oxygen species(ROS) levels were detected by green fluorescent probes;Intracellular antioxidant enzyme activity in HUVECs was assessed by the superoxide dismutase (SOD) and catalase (CAT) kits;flow cytometry was used to detect cell apoptosis rate;Western blot was used to detect the expression of Cleaved Caspase-3,B-cell lymphoma-2(BCL-2),and BCL-2 associated X protein (BAX) proteins. The results indicated that muscone (0. 9-9. 0 μg/ mL) had no effect on the growth of HUVECs. The optimal concentration of H₂O₂ for inducing oxidative damage in HUVECs was 400 μmol/ L for 2 hours. Compared with the H₂O₂ group,muscone (1. 125-4. 5 μg/ mL) inhibited the morphological changes of HUVECs induced by H₂O₂. Cell morphology was improved,cell viability was increased(P < 0. 001),the activities of SOD and CAT were enhanced(P < 0. 001,P < 0. 05),and the apoptosis rate and ROS levels were decreased by muscone(P < 0. 01,P < 0. 001). Western blot showed that the protein expressions of BCL-2 in muscone group were higher(P < 0. 01),while the expressions of Cleaved Caspase-3 and BAX were lower than that in H₂O₂ group (P < 0. 01,P < 0. 01). These findings suggested that muscone exhibited a protective effect against H₂O₂-induced HUVECs damage,potentially through the mechanisms involving the clearance of ROS,improvement of intracellular antioxidant enzyme activity,and inhibition of apoptosis.

Key words:

"> muscone, human umbilical vein endothelial cells, oxidative stress, apoptosis