基于UPLC 特征图谱和含量测定的白薇标准汤剂质量评价研究

doi:10.16333/j.1001-6880.2026.9.013

天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (9): 1988-1998.doi: 10.16333/j.1001-6880.2026.9.013 cstr: 32307.14.1001-6880.2026.9.013

基于UPLC 特征图谱和含量测定的白薇标准汤剂质量评价研究

李娇艳 1,2,刘晓霞 2,何民友 2,谢文凯 2,李振雨 2,陈向东 2,孙冬梅 2∗

1广州中医药大学,广州510006;2 广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244

出版日期:2026-09-24 发布日期:2026-09-22
基金资助:
2022 年佛山市南海区重点领域科技攻关专项(南科﹝2023 ﹞20 号-18)

Quality evaluation of Cynanchum atratum standard decoction based on UPLC characteristic spectrum and content determination

LI Jiao-yan1,2,LIU Xiao-xia2,HE Min-you2, XIE Wen-kai2,LI Zhen-yu2,CHEN Xiang-dong2,SUN Dong-mei2∗

1Guangzhou University of Chinese Medicine,Guangzhou 510006,China;2Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co. ,Ltd. ,Foshan 528244,China

Online:2026-09-24 Published:2026-09-22

摘要/Abstract

摘要：

建立白薇标准汤剂的特征图谱及对羟基苯乙酮和2,4-二羟基苯乙酮的含量测定方法,并通过特征图谱对白薇、蔓生白薇及老瓜头标准汤剂进行区分,以期对其质量进行评价。采用超高效液相色谱法(ultra-high performance liquid chromatography,UPLC)建立特征图谱,并通过聚类分析、主成分分析和正交偏最小二乘判别分析对15 批不同产地白薇饮片制备的标准汤剂进行化学模式识别分析。结果表明,不同产地白薇饮片制备的标准汤剂的UPLC 特征图谱确定了8 个共有峰,其中,峰5 为对羟基苯乙酮、峰7 为4-甲氧基-3-羟基苯乙酮、峰8 为2,4-二羟基苯乙酮。聚类分析将15 批样品按照产地分为4 类,与主成分分析、正交偏最小二乘判别分析的分类结果一致;正交偏最小二乘判别分析共筛选出3 个影响质量的共有特征峰,其中, 2,4-二羟基苯乙酮和对羟基苯乙酮被确定为定量分析指标。与白薇标准汤剂特征图谱相比,蔓生白薇的峰8 响应值接近基线,老瓜头则只有峰2 和峰3 两个色谱峰。建立的 UPLC 特征图谱和含量测定方法可用于不同产地白薇饮片制备的标准汤剂的质量控制,为白薇资源及其相关产品的质量评价提供了依据。

关键词:

"> 白薇, 标准汤剂, 超高效液相色谱法, 特征图谱, 含量测定, 化学计量学

Abstract:

This study aims to establish a characteristic fingerprint of the standard decoction of Cynanchum atratum and a method for the determination of P-hydroxyacetophenone and 2,4-dihydroxyacetophenone,and to distinguish the standard decoctions of C. atratum,C. versicolor,and C. komarovii by characteristic fingerprinting,so as to evaluate its quality. A characteristic fingerprint was established by ultra-high performance liquid chromatography (UPLC). Chemical pattern recognition analysis was performed on standard decoctions prepared from 15 batches of C. atratum slices from different origins using hierarchical cluster analysis (HCA),principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that eight common peaks were identified in the UPLC characteristic fingerprints of standard decoctions prepared from C. atratum slices from different origins. Among them,peak 5 was P-hydroxyacetophenone,peak 7 was 4-methoxy-3-hydroxyacetophenone,and peak 8 was 2,4-dihydroxyacetophenone. HCA divided the 15 batches of samples into four clusters according to their geographical origins,which was consistent with the classification results of PCA and OPLSDA. A total of three common characteristic peaks affecting quality were screened by OPLS-DA, among which 2, 4-dihydroxyacetophenone and P-hydroxyacetophenone were determined as the quantitative markers. Compared with the characteristic fingerprint of C. atratum standard decoction,the response value of peak 8 of C. versicolor was close to the baseline,while only peak 2 and peak 3 were observed in C. komarovii. The established UPLC characteristic fingerprint and content determination method can be used for the quality control of standard decoctions prepared from C. atratum slices from different origins, providing a scientific basis for the quality evaluation of C. atratum resources and their related products.

Key words:

"> Cynanchum atratum, standard decoction, UPLC, characteristic fingerprint, content determination, chemometrics

中图分类号:

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李娇艳, 刘晓霞, 何民友, 谢文凯, 李振雨, 陈向东, 孙冬梅. 基于UPLC 特征图谱和含量测定的白薇标准汤剂质量评价研究[J]. 天然产物研究与开发, 2026, 38(9): 1988-1998.

LI Jiao-yan, LIU Xiao-xia, HE Min-you, XIE Wen-kai, LI Zhen-yu, CHEN Xiang-dong, SUN Dong-mei. Quality evaluation of Cynanchum atratum standard decoction based on UPLC characteristic spectrum and content determination[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(9): 1988-1998.

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链接本文: http://www.trcw.ac.cn/CN/10.16333/j.1001-6880.2026.9.013

http://www.trcw.ac.cn/CN/Y2026/V38/I9/1988

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基于UPLC 特征图谱和含量测定的白薇标准汤剂质量评价研究

Quality evaluation of Cynanchum atratum standard decoction based on UPLC characteristic spectrum and content determination

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