微波改性竹炭颗粒对实验性腹泻大鼠免疫性能和消化吸收功能的影响

doi:A

摘要/Abstract

摘要： 旨以研究活性竹炭颗粒（MABCP）对实验性腹泻大鼠免疫性能和消化吸收功能的影响，在大鼠饲粮中分别添加0.05，0.10，0.15%的经KOH微波活化法处理竹炭颗粒，对模型大鼠进行为期一周的饲养试验。研究发现：经KOH微波活化的竹炭颗粒，比表面积与吸附性能大幅增强，其能显著降低腹泻大鼠稀便率（P<0.05），有效增加胸腺和脾脏指数（P<0.05）；显著提高腹泻大鼠血清D-木糖含量（P<0.05），但对消化酶如淀粉酶活性的分泌无显著影响（P>0.05）；肠道线粒体琥珀酸脱氢酶、NADH脱氢酶、H+-ATP酶活性显著增强（P>0.05），MDA生成量显著降低（P>0.05）。试验表明MABCP可提高体积免疫性能，改善小肠吸收功能，减少肠道自由基生成，有效维持或改善细胞氧化磷酸化水平，其中以低剂量添加效果更为明显。

关键词: 微波改性竹炭颗粒, 大鼠, 免疫性能, 消化吸收功能, 细胞氧化磷酸化

Abstract: In order to investigate the effect of bamboo charcoal particles on immune performance and digestive absorption function of experimentally induced diarrhea rats, microwave activated bamboo charcoal particles (MABCP) was added into their diets at 0.05%, 0.10%, and 0.15% to conduct a 7-day feeding trials. Microwave activation significantly enhanced the specific surface area and adsorption performance of bamboo charcoal particles. When MABCP were added into the rat diet, diarrhea rate was significantly reduced (P<0.05), and the thymus and spleen indices was effectively increased (P<0.05). Serum D-xylose content was significantly improved in the diarrhea rats (P<0.05), but not for digestive enzymes such as amylase activity (P>0.05). Intestinal mitochondrial NADH dedydrogenase, H+-ATPase and succinate dehydrogenase activities were increased (P<0.05) and MDA content was decreased significantly (P<0.05). The results indicated that MABCP could improve immune performance, enhance the small intestinal absorption function, decrease radical production and maintain/ameliorate cellular oxidative phosphorylation level; the comparatively optimal dietary level of MABCP was 0.05%.

Key words: microwave activated bamboo charcoal particles, rat, immune performance, digestive absorption function, cellular oxidative phosphorylation

中图分类号: TS231

王建辉，刘永乐，李赤翎，刘冬敏，尹欣欣，李艳，王群辉. 微波改性竹炭颗粒对实验性腹泻大鼠免疫性能和消化吸收功能的影响[J]. 天然产物研究与开发, 2012, 24(5): 677-681.

WANG Jian-hui, LIU Yong-le, LI Chi-ling, LIU Dong-min, YIN Xin-xin, LI Yan, WANG Qun-hui. Effect of Microwave Modified Bamboo Charcoal Particles on Immune Performance and Digestive Absorption Function of Experimentally Induced Diarrhea Rats[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 24(5): 677-681.

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