关键词: 加味苇茎汤, 含药血清, 芹菜素, A549, 周期阻滞, 细胞凋亡

Abstract: The objectives of this study were to investigate the inhibitory effect of self-made Jiawei Weijing Decoction and apigenin on the proliferation,cell cycle and apoptosis of lung adenocarcinoma A549 cell line and to explore its mechanism in the treatment of lung cancer.The experimental study used an inverted microscope observation of cell morphology.MTT assay was performed to reveal the inhibitory effect on cell proliferation and flow cytometry was used to determine the apotosis and cell cycle of lung adenocarcinoma A549 cell line after the treatment of self-made Jiawei Weijing Decoction and apigenin.Morphological changes of the apoptosis of cancer cells were observed by hochest 33342 staining.The results showed that compared with controls,the inhibition rates of self-made Jiawei Weijing decoction and apigenin on lung adenocarcinoma A549 cells were:in 24 hours group,31% and 34%,respectively;in 48 hours group,45% and 53%,respectively.Selfmade Jiawei Weijing decoction increased the rate of G2 of A549 cell cycle obviously and apigenin significantly induced the apoptosis of A549 cell.Hence,it was concluded that Self-made Jiawei Weijing decoction and apigenin inhibited the growth of human lung adenocarcinoma A549 cells and cause cell cycle arrest and induce apoptosis.

Key words: self-made Jiawei Weijing decoction, serum pharmacology, apigenin, A549, cell cycle arrest, apoptosis