天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (9): 1389-1392.

• 研究简报 • 上一篇    下一篇

苦竹内生真菌Phomopsis sp.KY-12化学成份及其海虾致死活性研究

郭芳1,杨胜祥1,2*,刘力1,2*,王勇2   

  1. 1 亚热带森林培育国家重点实验室;2 浙江省生物质化学利用重点实验室,临安 311300
  • 出版日期:2014-09-30 发布日期:2014-11-02

Chemical Constituents and Their Toxic Activity from the Endophytic Fungus Phomopsissp. KY-12, Isolated from Pleioblastus amarus

GUO Fang1, YANG Sheng-xiang1,2*, LIU Li1,2*, WANG Yong2   

  1. 1 The Nurturing Station for the State Key Laboratory of Subtropical Silviculture; 2 Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, Zhejiang A & F University, Lin’an 311300, China
  • Online:2014-09-30 Published:2014-11-02

摘要: 利用柱色谱技术从苦竹内生真菌中分离得到13个化合物,包括九个杜松烷型倍半萜、一个azaphilone类化合物和三个甾体类化合物,经波谱鉴定为3,12-dihydroxycalamenene (1), 3,9,12-trihydroxycalamenene (2), indicumolide C (3), agripilol C (4), 2,15-tihydroxycalamenene (5), dysodensiol D (6), bombamalones D (7), 8-formyl-7-hydroxyl-5-isopropyl-2-methoxy-1,4-naphthoquinone (8), strobilol A (9), Pyrenocine J (10), 3β, 5α-Dihydroxy-6β-methoxyergosta-7,22-dien (11),麦角甾醇(12)和麦角甾醇过氧化物(13)。其中,化合物3~9为首次从该属真菌中分离得到。化合物1~8显示中等程度的细胞毒活性,化合物9 显示显著的细胞毒活性。

关键词: 内生真菌, 杜松烷型倍半萜, 苦竹, 细胞毒活性, Phomopsis sp.

Abstract: To study the chemical constituents and their toxic activity from the endophytic fungus Phomopsis sp. KY-12, isolated from Pleioblastus amarus. The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Cytotoxic activity was performed by the brine shrimp (Artemiasalina) larvae bioassay.13known compounds including 9Cadinanesesquiterpenoids, 1 azaphilone and 3 steroids,named 3,12-dihydroxycalamenene (1), 3,9,12-trihydroxycalamenene (2), indicumolide C (3), agripilol C (4), 2,15-tihydroxycalamenene (5), dysodensiol D (6), bombamalones D (7), 8-formyl-7-hydroxyl-5-isopropyl-2-methoxy-1,4-naphthoquinone (8), strobilol A (9), Pyrenocine J (10), 3β, 5α-Dihydroxy-6β-methoxyergosta-7,22-dien (11), ergosterol (12) and ergosterol peroxide (13),were isolated from the organic extract of fermentation broths of the endophytic fungus, Phomopsis sp. KY-12. Compounds 3-9 were first isolated from the Phomopsis genus.Compounds 1-8 showed moderate active against the brine shrimp larvae,compound 9 indicated significant growth inhibitory activity against the brine shrimp (Artemias alina) larvae.

Key words: Endophytic fungus, Phomopsis sp.KY-12;Cadinane sesquiterpenoids;toxic activity;Pleioblastus amarus

中图分类号: 

R284.2