天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 110-113.

• 开发研究 • 上一篇    下一篇

黍稷麸皮酚酸提取物抗氧化活性研究

牛伟1*,鹿茸2,乔治军3,张丽珍2   

  1. 1山西省农业科学院;2 山西大学生命科学学院;3 农业部黄土高原作物基因资源与种质创制重点实验室 山西省农业科学院农作物品种资源研究所,太原 030006
  • 出版日期:2014-12-30 发布日期:2015-03-04

Antioxidant Activity of Phenolics from Panicum miliaceum L.Bran

NIU Wei1*, LU Rong2, QIAO Zhi-jun3, ZHANG Li-zhen2   

  1. 1 Shanxi Academy of Agricultural Sciences;2 School of Life Science,Shanxi University;3 Key Laboratory aiyuan of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030006,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 以黍稷麸皮Cimi2为研究对象,比较了四种多酚提取方法的效果及提取物清除自由基的能力。从总酚来看,用乙醇浸提法、超声助溶法、酶解法以及超声与酶结合法分别得到225.77、192.23、231.01、221.15 mg没食子酸/100 g样品。四种方法中得到的结合酚和自由酚比例为49:51、29:71,31:69、 28:72。对9个品种的麸皮提取物的体外抗氧化能力检测结果表明,黍稷麸皮酚酸对DPPH、超氧阴离子、羟自由基都有明显的清除作用。其中zigangmi清除DPPH自由基能力和体外总抗氧化能力最强最强,Cimi2的清除羟自由基能力最强。

关键词: 黍稷, 自由酚, 结合酚, 提取方法, 抗氧化能力

Abstract: The bran of proso millet cultivars were analyzed for their phenolic content and antioxidant activities.Four extraction methods,including ethanol solvent、ultrasonicassisted ethanol extraction、enzymolysis、ultrasonicenzymolysis method,were compared for their efficiency of phenolic content of the Cimi2 bran.The total phenolic content were 225.77、192.23、231.01、221.15 mg/100 g respectively.The ratios of bound phenolic to free phenolic were 49:51、29:71、31:69、28:72 for four extraction methods respectively.Total phenolics from proso millet bran has a scavenging activity on DPPH、O2-· and ·OH radicals.The highest DPPH scavenging ability and total antioxidant capacity was found in zigangmi bran.The highest hydroxyl radical scavenging ability was found in Cimi2 bran.

Key words: Panicum miliaceum L.,free phenolic, bound phenolic, extraction methods, antioxidant activity

中图分类号: 

R284.2