关键词: 红叶李花, 闪式, 酶, 多糖, 响应面设计, 单糖分析

Abstract: The aim of this study were to optimize the process of enzymatic coupled with smashing tissue extraction and purification of polysaccharides from the flowers of Prunus cerasifera Ehrh.cv. Atropurpurea Jacg and to analyze their monosaccharide compositions by GC-MS.Based on single-factor tests,response surface design was used to assure the optimal process.Polysaccharides which were separated by DEAE52 cellulose column and Sephadex G-150 chromatography and treated by acetylation was determined by GC-MS. The results showed the optimal extraction parameters as follows:enzymatic temperature was 45 ℃,enzymatic time was 2 h,enzymatic dosage was 0.17%,extraction time was 3 min,liquidsolid ratio was 35:1,revolution speed was 3000 rpm.Under the optimized conditions,the yield of polysaccharides yield 13.02%.Polysaccharides were composed of PPCS-I and PPCS-II,in which PPCS-I contained Rhamnose, Arabinose, Xylose, Mannose,and Glucose with a molar ratio of 12.11:3.16:7.06:1:4.92,PPCS-II contained Rhamnose,Xylose,Mannose,Glucose and Galactose with a molar ratio of 5.02:1:13.65:11.76:8.39.

Key words: Prunus cerasifera Ehrh.cv.Atropurpurea Jacg., smashing tissue, enzyme, polysaccharides, response surface design, monosaccharide analysis