天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (5): 651-654.

• 研究论文 • 上一篇    下一篇

树舌灵芝的化学成分及新化合物的绝对构型测定(补充数据)

卜庆华1,2#,罗奇2#,程永现2*   

  1. 1 大理大学药学与化学学院,大理 671002;2 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明 650201
  • 出版日期:2016-05-28 发布日期:2016-09-06

Chemical Constituents from Ganoderma applanatum and Absolute Configuration Determination of New Compounds

BU Qing-hua1,2#,LUO Qi2#,CHENG Yong-xian2*   

  1. 1 College of Pharmaceutical Science,Dali University,Dali 671002,China; 2 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China
  • Online:2016-05-28 Published:2016-09-06

摘要: 利用各种色谱技术从树舌灵芝的子实体中分离得到8个化合物,结合波谱学方法鉴定了它们的结构,分别为:shushene A (1)、shushene B(2)、lucidulactone A(3)、2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(4)、6-hydroxy-4-H-chromen-4-one(5)、1-(4-ethylphenyl)-1,2-ethanediol(6)、1-(2-ethylphenyl)-1,2-ethanediol(7)和吲哚醛(8)。其中化合物1和2是新化合物,化合物3~8系首次从树舌灵芝中获得。化合物1和2是外消旋体,我们对其进行了手性拆分并通过量子化学计算确定了其对映体的绝对构型。此外,还采用ELISA法对化合物1和2的肾保护活性进行了测试。

Abstract: Eight compounds including two new compounds,namely shushenes A (1) and B (2),were isolated from the fruiting bodies of Ganoderma applanatum. Their structures were identified by means of spectroscopic methods.The structures of the known compounds are lucidulactone A (3),2,3-dihydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-1-propanone (4),6-hydroxy-4-H-chromen-4-one (5),1-(4-ethylphenyl)-1,2-ethanediol (6),1-(2-ethylphenyl)-1,2-ethanediol (7) and indole-3-carboxaldehyde (8),which were all isolated from this species for the first time.Of note,compounds 1 and 2 were isolated as racemic mixtures,chiral separation followed by computational methods allowed their absolute configuration assignment.In addition,inhibition of compounds 1 and 2 on fibronectin expression was tested by using an enzyme-linked immunosorbent assay (ELISA).