Turpiniae Folium is widely used as a functional food or medicine in China.However,little investigations are performed on the chemical composition and biological activity of its volatile oil.In this work,Turpiniae Folium oil extracts (Supercritical extraction) were systematically characterized for the first time.The low polar substances were identified by GC-MS (methyl esterification and non-methylesterification),and LC-MS method was applied to conduct analysis of the intermediate polar substances.Also,the anti-oxidant (DPPH and ABTS method) and anti-inflammatory (LPS-induced RAW 264.7 cell model) activity of the oil from Turpiniae Folium were performed.80 chemical components were identified by GC-MS (38 for non-methyl esterification and 42 for methyl esterification,respectively),most of which were linoleic acid,γ-sitosterol,squalene,2,2′-methylenebis-(4-methyl -6-tert-butylphenol),trans-9-octadecenoic acid,palmitic acid and other substances.In the positive and negative ion mode of LC-MS,a total of 256 compounds were identified,most of which were terpenes,phenylpropanoids,phenols,flavonoids,alkaloids,coumarins and their derivatives,fatty acyls,semi-terpenoids,fatty acids and other compounds.Moreover,the volatile oil from Turpiniae Folium had good ability to resist DPPH and ABTS free radicals,and could significantly inhibit the release of NO and IL-6 in RAW 264.7 cells induced by LPS in a dose-dependent manner.Generally,these results proved the anti-bacterial,anti-inflammatory,and immunoregulatory effects of the volatile oil from Turpiniae Folium,thus providing a reference for the application of PVO as a food or drug.