天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (9): 1507-1514.doi: 10.16333/j.1001-6880.2022.9.007

• 研究简报 • 上一篇    下一篇

垫状卷柏中化学成分的分离及其抑制肿瘤细胞增殖活性

蔡   蕊1，于远洋2，谢   宁3，徐   强1，万慧慧1，王世盛3,4*    

  1. 1大连理工大学分析测试中心，大连 116024；2大连市检验检测认证技术服务中心，大连 116081；3大连理工大学化工学院，大连 116024；4大连理工大学宁波研究院，宁波 315016
  • 出版日期:2022-09-28 发布日期:2022-10-08
  • 基金资助:
    中央高校基本科研业务费（DUT21YG124）

Isolation of chemical constituents from Selaginella pulvinata and their anti-proliferative activity on tumor cells

CAI Rui1,YU Yuan-yang2,XIE Ning3,XU Qiang1,WAN Hui-hui1,WANG Shi-sheng3,4*   

  1. 1 Analytical Instrumentation Center,Dalian University of Technology,Dalian 116024,China;2Dalian Inspection and Certification Technical Service Center,Dalian 116081,China;3Department of Pharmaceutical Sciences,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,China;4Ningbo Institute of Dalian University of Technology,Ningbo 315016,China
  • Online:2022-09-28 Published:2022-10-08

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摘要：

利用质谱引导的自动纯化分离系统对垫状卷柏95%乙醇提取物的乙酸乙酯萃取部分进行选择性分离纯化双黄酮类、炔酚类化合物。并利用MS、1H NMR、13C NMR、2D NMR等光波谱分析方法确定结构，结果得到四个双黄酮类化合物，分别为扁柏双黄酮（1）、异柳杉双黄酮（2）、苏铁双黄酮（3）、穗花杉双黄酮（4），七个炔酚类化合物，分别为selaginellin（5）、selaginpulvilin A（6）、selaginpulvilin B（7）、selaginellin O（8）、selaginellin E（9）、selaginellin A（10）、selaginellin B（11）。采用MTT法评价了化合物1~11体外抑制人非小细胞肺癌细胞（NSCLC） H322增殖活性，结果表明，除化合物4和7外，其他化合物对人非小细胞肺癌细胞H322有不同程度的增殖抑制活性，其中化合物3和10抑制作用较强，IC50值分别为16.70和9.54 μmol/L。

关键词: 垫状卷柏, 分离纯化, 双黄酮, 炔酚, 肿瘤细胞增殖抑制

Abstract:

Targeting isolation of biflavonoids and selaginellin derivatives from Selaginella pulvinata (Hook.& Grev.) Maxim. was investagated by was performed on a Waters preparative LC-MS autopurification system and the ethyl acetate part of the 95% ethanol extract of S. pulvinata was separated and purified. The chemical structures of the separated compounds were identified by MS,1H NMR,13C NMR,2D NMR and spectral analysis methods. Four biflavonoids were isolated and identified as hinokiflavone (1),isocryptomerin (2),sotetsuflavone (3) and amentoflavone (4). Seven selaginellin derivatives were obtained and identified as selaginellin (5),selaginpulvilin A (6),selaginpulvilin B (7),selaginellin O (8),selaginellin E (9),selaginellin A (10) and selaginellin B (11). The MTT assay was used to evaluate the inhibitory effect of compounds 1~11 on the proliferation of human non-small cell lung cancer cell line (NSCLC) H322 in vitro. The results showed that compounds 1,2,3,5,6,8,9,10 and 11 had different degrees of inhibitory activity on human NSCLC cell line H322. Compounds 3 and 10 had stronger inhibitory activity on human NSCLC cell line H322,with IC50 values of 16.70 μmol/L and 9.54 μmol/L,respectively.

Key words: Selaginella pulvinata (Hook. &, Grev.) Maxim., isolation and purification, biflavonoids, selaginellins, anti-proliferative activity on tumor cells

中图分类号:  R932

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