关键词: 甘松, 不同部位, 挥发油, 甘松新酮, 绿原酸, 总黄酮

Abstract:

This study aims to investigate the differences of main components in different parts of Nardostachys jatamansi DC.and dig its potential value.The content of volatile oil in different parts of N.jatamansi were determined by steam distillation,the content of nardosinone,chlorogenic acid,and total flavonoids in different parts were determined by high performance liquid chromatography and ultraviolet-visible spectroscopy,the volatile components in different parts were analyzed by gas chromatography-mass spectrometry,and the key volatile differential components were identified by orthogonal partial least-squares discrimination analysis (OPLS-DA).The results showed that the content of volatile oil (6.00%) and nardosinone (49.0 mg/g) were the highest in roots,and the content of total flavonoids (46.7 mg/g) and chlorogenic acid (17.4 mg/g) were the highest in stems.A total of 53 volatile components were identified from different parts,including 47 from roots,35 from stems,46 from leaves,and 50 from flowers.Sesquiterpenes were the main volatile components from different parts.The relative content of sesquiterpenes was the highest in roots,the relative content of aldehydes and acids were the highest in stems,the relative content of phenols,monoterpenes,ketones and others were the highest in leaves,and the relative content of esters and alcohols were the highest in flowers.The OPLS-DA results showed that there were differences in volatile components in different parts,and 13 key volatile components such as ylangenal,calarene,and hexadecanoic acid were screened out by variable importance in projection results.In conclusion,there are differences in the main components of different parts from N.jatamansi,which can provide a scientific basis for the further excavation and utilization of different parts of N.jatamansi.

Key words: Nardostachys jatamansi DC., different parts, volatile oil, nardosinone, chlorogenic acid, total flavonoids