关键词:

"> 爪哇木棉, 化学成分, 细胞毒活性, 杜松烷型倍半萜

Abstract:

To investigate the chemical constituents and anti-tumor activities of the pharmacological effects of Ceiba pentandra L. ,the study was carried out to isolate and purify the chemical components from the ethyl acetate extract of the stem using silica gel and semi-preparative high-performance liquid chromatography and identify the structures of the compounds by modern spectroscopic methods such as NMR,MS,and so on. Meanwhile,the cytotoxic activities of all compounds against BT549 and 4T1 tumor cells were evaluated using the CCK-8 assay. Nineteen compounds were isolated and identified as:hibiscusterpene II (1),hibiscone C (2),7-hydroxycadalene (3),7-hydroxycalamenene (4),(1S)-1-methoxylacinilene C(5),lacinilene C (6),(7R,9R,10R)-3,9-di-hidroxicalameneno (7),mansonone (8),3ξ-(1ξ-hydroxyethyl)-7-hydroxy-1-isobenzofuranone (9),vavain (10),cleomiscosin B(11),( + )-medioresinol (12),1-methoxy-2-methylbenzene (13),p-cresol (14),syringaldehyde (15),(R)-(-)-mellein methyl ether (16),chrysazin (17),ethyl syringate (18) and β-sitosterol (19). Compounds 1,2,4-9,11-18 were isolated for the first time from this genus. The results showed that compounds 1,2,3,4,6,8,and 10 exhibited inhibitory activity against the proliferation of both BT549 and 4T1 cells,among which compounds 4 and 8 demonstrated significant cytotoxicity. This study systematically elucidates for the first time that cadinane-type sesquiterpenoids are the key antitumor constituents in C. pentandra,thereby providing a scientific basis for its further application in anticancer drug development.

Key words:

"> Ceiba pentandra L., chemical constituents, cytotoxic activities, cadinane-type sesquiterpene