基于谱效关系的吴茱萸酒制前后治疗痛经的药效物质基础研究

doi:10.16333/j.1001-6880.2026.9.014

天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (9): 1999-2007.doi: 10.16333/j.1001-6880.2026.9.014 cstr: 32307.14.1001-6880.2026.9.014

基于谱效关系的吴茱萸酒制前后治疗痛经的药效物质基础研究

刘叶倩,龙红萍,黄玲格,冯敏,龚姗,陈春茗,任卫琼 ∗

湖南中医药大学第一附属医院,长沙410007

出版日期:2026-09-24 发布日期:2026-09-22
基金资助:
湖南省中医药管理局科研项目(B2024043);湖南中医药大学校院联合基金(2025XYLH019)

Pharmacodynamic material basis of Euodiae Fructus before and after wine-processed in the treatment of dysmenorrhea based on spectrum-effect relationship

LIU Ye-qian,LONG Hong-ping,HUANG Ling-ge, FENG Min,GONG Shan,CHEN Chun-ming,REN Wei-qiong∗

The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China

Online:2026-09-24 Published:2026-09-22

摘要/Abstract

摘要：

基于谱效关系探究吴茱萸(Euodiae Fructus)酒制前后治疗痛经的药效物质基础。利用UPLC-Q-TOF-MS 技术分析大鼠灌胃吴茱萸生品、酒制品后的入血成分。建立痛经大鼠模型,以扭体次数、扭体潜伏期、肿瘤坏死因子-α (tumor necrosis factor-α,TNF-α)、前列腺素E2(prostaglandin E2,PGE2)、PGF2α 为指标评价其治疗痛经的作用;运用灰色关联度和熵权法分析吴茱萸酒制前后入血成分与治疗痛经作用的谱效关系。结果从不同批次的吴茱萸生品和酒制品血清样品中检测到12 个共有峰,并鉴定出5 个入血成分;吴茱萸能减少痛经大鼠扭体次数、延长扭体潜伏期 (P < 0. 01),降低血清中PGF2α、TNF-α 含量,上调PGE2 水平(P < 0. 05 或P < 0. 01),吴茱萸酒制品的效果较生品强 (P < 0. 05 或P < 0. 01);谱效关联结果表明,酒制后7-羟基香豆素、柠檬苦素的关联度综合得分明显上升,且与酒吴茱萸治疗痛经疗效增强呈正相关。本研究初步明确了吴茱萸酒制前后治疗痛经的体内药效物质基础,为酒吴茱萸的临床应用提供了实验依据。

关键词:

"> 吴茱萸, 入血成分, 酒制吴茱萸, UPLC-Q-TOF-MS, 谱效关系

Abstract:

The study aims to investigate the pharmacodynamic material basis of Euodiae Fructus before and afte wine-processed in treating dysmenorrhea based on spectrum-effect relationship. UPLC-Q-TOF-MS was used to analyze the blood-absorbed components of Euodiae Fructus and its wine-processed product. The dysmenorrhea rat model was established. The treatment of dysmenorrhea was evaluated using the writhing times,writhing latency,tumor necrosis factor-α (TNF-α),prostaglandin E2 (PGE2) and PGF2α as indicators. Grey relational degree and entropy weight method were applied to analyze the spectrum-effect relationship between blood-absorbed components and analgesic efficacy. The results showed that 12 common peaks were detected from these serum samples of different batches Euodiae Fructus and its wine-processed product,and 5 of these common peaks were identified. Euodiae Fructus effectively reduced the writhing times,prolongated the writhing latency (P < 0. 01),downregulated the contents of serum PGF2α and TNF-α,and upregulated the level of PGE2 (P < 0. 05 or P < 0. 01). Wine-processed Euodiae Fructus had better than Euodiae Fructus (P < 0. 01). The results of spectrum-effect relationship indicated that the correlation degree score of 7-hydroxycoumarin and limonin significantly increased after wine-processed,and show positive correlation with the treatment of dysmenorrhea after wine-processed. This study initially clarified the pharmacodynamic material basis of Euodiae Fructus before and after wine-processed in the treatment of dysmenorrhea,which provided an experimental basis for the clinical application of wine-processed Euodiae Fructus.

Key words:

Euodiae Fructus, blood-absorbed components, wine-processed Euodiae Fructus, UPLC-Q-TOF-MS, spectrumeffect relationship

中图分类号:

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刘叶倩, 龙红萍, 黄玲格, 冯敏, 龚姗, 陈春茗, 任卫琼. 基于谱效关系的吴茱萸酒制前后治疗痛经的药效物质基础研究[J]. 天然产物研究与开发, 2026, 38(9): 1999-2007.

LIU Ye-qian, LONG Hong-ping, HUANG Ling-ge, FENG Min, GONG Shan, CHEN Chun-ming, REN Wei-qiong. Pharmacodynamic material basis of Euodiae Fructus before and after wine-processed in the treatment of dysmenorrhea based on spectrum-effect relationship[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(9): 1999-2007.

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链接本文: http://www.trcw.ac.cn/CN/10.16333/j.1001-6880.2026.9.014

http://www.trcw.ac.cn/CN/Y2026/V38/I9/1999

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基于谱效关系的吴茱萸酒制前后治疗痛经的药效物质基础研究

Pharmacodynamic material basis of Euodiae Fructus before and after wine-processed in the treatment of dysmenorrhea based on spectrum-effect relationship

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