天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 964-970.doi: 10.16333/j.1001-6880.2021.6.009

• 研究简报 • 上一篇    下一篇

核桃分心木镇静催眠活性成分研究

洪茜茜1，耿树香2，张银志1，孙秀兰1，徐德平1*   

  1. 1江南大学食品学院，无锡 214122；2云南省林业科学院，昆明 650201
  • 出版日期:2021-06-28 发布日期:2021-07-02
  • 基金资助:
    云南省重大科技专项计划（2018ZG003）

Study on the sedative and hypnotic active components of Diaphragma Juglandis Fructus

HONG Qian-qian1,GENG Shu-xiang2, ZHANG Yin-zhi1,SUN Xiu-lan1,XU De-ping1*   

  1. 1School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;2Yunnan Academy of Forestry,Kunming 650201,China

  • Online:2021-06-28 Published:2021-07-02

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摘要： 探究核桃分心木镇静催眠功效成分，为分心木应用于镇静催眠类保健食品或者药品提供理论依据。采用乙醇和水提取，多种层析分离，通过小鼠镇静催眠行为学实验，筛选分心木镇静催眠活性成分，利用核磁共振鉴定化学结构。结果表明，分心木提取物经大孔树脂分离得的水洗脱物具有显著的镇静催眠作用，再经MCI、ODS等柱分离，从该部分得到4个单体化合物，分别是2-羟甲基-四氢吡喃-3，4，5-三醇（1）、3-羟基-4-亚氨基丁酸（2）、（1′-甲基-2′-羟基）丙烷-O-α-D-吡喃葡萄糖苷（3）、6-羟基萘基-1，2-（2-O-β-D-吡喃葡萄糖苷）（4）。其中化合物1仅能显著延长小鼠睡眠时间，而化合物2则可显著降低小鼠运动速度和时间，增加在中央区域停留的时间，且可缩短潜伏期，延长睡眠时长。分心木是潜在镇静催眠的天然活性产物，在保健食品药品领域的研究与应用具有重要研究意义和开发价值。


关键词: 核桃分心木, 成分分离, 结构鉴定, 镇静催眠, 3-羟基-4-亚氨基丁酸

Abstract:

Explore the sedative and hypnotic components of Diaphragma Juglandis Fructus(DJF),and provide a theoretical basis for the application of DJF in sedative and hypnotic health products or medicines.The active constituents were extracted by ethanol and water,separated and purified by multiple chromatography,and then identified the chemical structure by NMR.The results showed that the water eluate separated by the macroporous resin from the extract of DJF had a significant sedative and hypnotic effect,and then separated by MCI,ODS and other columns.From this part,four monomer compounds are obtained,respectively 2-(hydroxymethyl) oxane-3,4,5-triol (1),3-hydroxy-4-iminobutanoic acid (2),(1′-methyl-2′-hydroxy) propane-O-α-D-glucopyranoside (3),6-hydroxy naphthyl-1,2-(2-O-β-D-pyran glucoside) (4).Compound 1 could only significantly prolong the sleep time of mice,while compound 2 could significantly reduce the mouse's movement speed and time,increase the time spent in the central area,and could shorten the incubation period and prolong the sleep duration.DJF is a natural active product with potential sedative and hypnotic,and its research and application in the field of health care has important research significance and development value.

Key words: Diaphragma Juglandis Fructus, component separation, structure identification, sedative-hypnotic effect, 3-hydroxy-4-iminobutanoic acid

中图分类号:  R284.2

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