The objective of this study was to examine the impact of salvianolic acid B (Sal B) on liver injury in mice,as well as its effects on intestinal flora and short-chain fatty acids (SCFAs).To establish a mouse liver injury model,a subcutaneous injection of 20% CCl₄ solution was administered.At the same time,mice in treatment groups were subjected to Sal B and the positive drug 2-deoxy-D-glucose gavage intervention,respectively.After the administration,the pathological changes of liver tissue were observed by hematoxylin-eosin (HE),Masson,and Sirius red staining,and the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by biochemical kits.16S rRNA sequencing technology was used to detect the changes of intestinal microbiota in mice.Additionally,the contents of SCFAs in mice feces were determined through gas chromatography.The results indicate that Sal B has a significant effect on reducing serum ALT and AST levels (P < 0.001) and mitigating liver pathological damage.Moreover,Sal B was revealed to partly restore the composition of intestinal flora in mice with liver injury,as evidenced by the sequencing data.Furthermore,the administration of Sal B resulted in a significant increase in the concentrations of isobutyric acid,isovaleric acid,propionate acid,and valeric acid in the feces from mice with liver injury(P < 0.001).Consequently,the above results could conclude that Sal B possesses the ability to alleviate liver injury induced by CCl₄ in mice.The mechanism is potentially related to its regulation of the balance of intestinal flora and the restoration of the contents of SCFAs.