天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (增刊1): 1-12.

• 研究论文 •    下一篇

酸橙黄酮和生物碱类物质生物合成的转录组分析

潘   媛1,2,3，谭   均1,2,3，赵   晓1,2,3，吕   卉1,2,3，杨仕春4*，陈大霞1,2,3*   

  1. 1重庆市中药研究院；2中国中医科学院中药资源中心重庆分中心；3重庆市中药良种选育与评价工程技术研究中心；4重庆市江津区多种经营技术推广中心，重庆 400065
  • 出版日期:2024-09-14 发布日期:2024-07-17
  • 基金资助:
    重庆市江津区现代农业产业技术体系首席专家项目和英才计划（〔2022〕23号）；重庆市英才计划“创新领军人才”（CQYC20200301170）

Transcriptome analysis of biosynthesis of flavonoid and alkaloid from Citrus aurantium L.

PAN Yuan1,2,3,TAN Jun1,2,3,ZHAO Xiao1,2,3,LYU Hui 1,2,3,YANG Shi-chun4*,CHEN Da-xia1,2,3*   

  1. 1Chongqing Academy of Chinese Material Medica;2Chongqing Sub-center of National Resource,Center for Chinese Materia Medica,China Academy of Chinese Medical Science;3Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica;4Jiangjin District of Chongqing City Variety of Management Technology Promotion Center,Chongqing 400065,China
  • Online:2024-09-14 Published:2024-07-17

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摘要：

本研究采用高通量转录组技术平台Illumina HiSeq 4 000构建不同发育时期酸橙果实转录组数据库，挖掘酸橙生物碱与黄酮类成分生物合成关键酶基因，在分子水平上进一步研究其药用成分的生物合成。经Trinity组装获得133 155条Unigeness，平均长度1 892 bp。通过KEGG代谢通路分析，发现酸橙转录组中有2 640条Unigeness参与到13条次生代谢产物生物合成相关的代谢通路。进一步分析参与酸橙生物碱和黄酮类生物合成相关基因，筛选获得9条参与生物碱和黄酮类生物合成的关键酶基因。本研究首次从两类成分入手探讨酸橙有效成分生物合成动态转录变化，丰富了酸橙的遗传信息，为进一步解析酸橙药用成分生物合成的调控机制提供科学依据。

关键词: 酸橙, 黄酮类, 生物碱类, 转录组

Abstract:

Illumina HiSeq 4000 technology platform was used to construct the transcriptome database of Citrus aurantium L. at different developmental stages,and the key enzyme genes for the biosynthesis of alkaloids and flavonoids were mined to further study the biosynthesis of the medicinal components at the molecular level.A total of 133 155 Unigeness with an average length of 1 892 bp were obtained by Trinity assembly.According to KEGG metabolic pathway analysis,2 640 Unigeness were found to be involved in 13 metabolic pathways related to the biosynthesis of secondary metabolites.The genes involved in the biosynthesis of alkaloids and flavonoids were further analyzed,and nine key enzyme genes involved in the biosynthesis of alkaloids and flavonoids were screened.In this study,the dynamic transcriptional changes of active components biosynthesis were studied from two components for the first time,which enriched the genetic information and provided scientific basis for further analysis of the regulatory mechanism of biosynthesis of medicinal components of C. aurantium.

Key words: Citrus aurantium L., flavonoid, alkaloid, transcriptome

中图分类号:  R932

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