天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (4): 486-489.doi: A

• 研究简报 • 上一篇    下一篇

应用高速逆流色谱分离桑枝酚类成分

罗 隽1,2，孙培文1,2，刘 韶1,2，李新中1,2*   

  1. 1中南大学湘雅医院药剂科，长沙 410008；2中南大学药学院，长沙410010
  • 收稿日期:2010-10-11 出版日期:2012-04-26 发布日期:2012-05-16

Separation of Phenols from Ramulus mori. by High-Speed Counter-Current Chromatography

LUO Jun1,2, SUN Pei-wen1,2, LIU Shao1,2, LI Xin-zhong1,2*   

  1. 1Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China; 2School of Pharmacy, Central South University, Changsha 410010, China
  • Received:2010-10-11 Online:2012-04-26 Published:2012-05-16

摘要： 建立了高速逆流色谱（HSCCC）分离制备高纯度的桑枝酚类成分的新方法。分离条件如下：溶剂系统为正己烷-乙酸乙酯-甲醇-水（1:1:1:2，v/v），上相为固定相，下相为流动相；流速2.0 mL/min；转速900 r/min；进样量75 mg。收集得到三个高纯度化合物，经HPLC、MS、1H和13C NMR等分别鉴定为反式氧化白藜芦醇（25.2 mg），反式白藜芦醇（7.4 mg）和桑辛素 M（29.1 mg）。高速逆流色谱可以高效分离桑枝成分，方法简便，技术可行，优于传统的柱色谱法。

关键词: HSCCC, 桑枝, 酚类, 氧化白藜芦醇, 白藜芦醇, 桑辛素 M

Abstract: A new method was developed for the separation and purification of phenols by high-speed counter-current chromatography (HSCCC) from extract of Ramulus mori. The separation condition was determined as follows: n-hexane: ethyl acetate: methyl: water (1:1:1:2,v/v) as the solvent system, the upper phase as the stationary phase, the lower phase as the mobile phase, 2.0 mL/min of flow-rate, 900 rpm of rotation speed, and 75 mg of injection amout. Trans-oxyresveratrol (25.2 mg), trans-resveratrol (7.4 mg), and moracin M (29.1 mg) were obtained and identified by HPLC, MS, 1H and 13C NMR. It indicated that HSCCC can be used for separating the active compounds of Ramulus mori efficiently and exhibited many advantages compared with the column chromatography method.

Key words: HSCCC, Ramulus mori., phenols, oxyresveratro1, resveratrol, moracin M

中图分类号: 

R284.2

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