A new diterpene quinone wiltriptobenzene (1) and nineteen known ones,including triptoquinone H (2),triptoquinone B (3),hinokione (4),triptonoterpene (5),triptonediol (6),triptobenzene A (7),wilforol F (8),triptobenzene B (9),abietatrien-3β-ol (10),triptobenzene S (11),hypoglicin B (12),triregelin H (13),triptophenolide (14),neotriptophenolide (15),16α-hydroxy-19,20-epoxy-19R*-methoxy-kaurane (16),16α-hydroxy-19,20-epoxy-20R*-ethoxy-kaurane (17),16α-hydroxy-19,20-epoxy-19R*-ethoxy-kaurane (18),fischericin D (19),and ent-pimara -8(14),15-diene-19-ol (20) were isolated from the ethyl acetate extract of Tripterygium wilfordii by silica gel,medium pressure liquid chromatography (MPLC),Sephadex LH-20,and HPLC chromatograph.The structures of them were established by extensive spectroscopic methods and comparing the data with those in the literature.Compounds 10,19,and 20 were isolated from T. wilfordii for the first time．The anti-inflammatory of activity of 1~20 was evaluated in LPS-induced RAW 264.7 cells.Anti-tumor activity of 1-20 in vitro against HeLa cell line was examined by MTT method.Compounds 2,3,and 20 exhibited significant activity to inhibit NO production with IC₅₀ values of 2.01,1.70 and 1.77 μmol/L.Compound 11 exhibited weak anti-tumor activity (IC₅₀ = 18.41 μmol/L).