Pseudomonas aeruginosa promotes the expression of a variety of virulence factors and develops drug resistance with the help of the quorum sensing system in vivo,which has become a major problem in the treatment of clinical infections,and it is extremely urgent to find new antibacterial drugs.2 733 common Chinese herbal medicines in Guangxi were selected in this work,and the active constituent library was established.After that,five important drug-resistant relative proteins in the quorum sensing were selected,and then they were docked with molecules,excepting to screen Chinese herbal medicine.Finally,the results showed that 17 ligands could be screened and 7 corresponding Chinese herbs could be applied as the potential quorum sensing inhibitors:Galla Chinensis,Rhizoma et Radix Boehmeriae,Radix Bauhiniae Purpureae,Herba Polygoni Chinensis,Herba Phyllanthi Urinarise,Folium Glyptostrobi Pensilis,Herba Melastomatis Polyanthi,respectively.The effectiveness of the selected traditional Chinese medicine was proved by the antimicrobial test of drug-resistant strains.