Abstract: To isolate endophytic fungus from Uncaria scandens and test monoamine oxidase (MAO) inhibitory and acetylcholinesterase (AChE) inhibitory activity of extracts form endophytic fungus.And the chemical constituents of compounds with high inhibitory activity were identified.Entophytic fungus were isolated by issue-culture method.The extracts were obtained by fermentation,extraction,methanol refluxing etc.The MAO inhibitory and the AChE inhibitory activity of extracts were tested by colorimetric method in 96-well microplates.The structures of compounds were identified by various chromatography methods.9 strains of endophytic fungus were isolated from Uncaria scandens.Three extracts showed anti-AChE activity with inhibitory rate more than 50%.The active strain QDE-6 was identified through molecular biological characterization and morphological methods,it is Aspergillus sp..5 compounds were isolated from endothytic fungi QDE-6 and identified as Aspergiketone(1)、Gliotoxin(2)、Pseurotin A₂(3)、Brevianamide F(4)、Pseurotin A₁(5).The inhibitory rate of compound 3 was 51.67%,which showed higher anti-MAO activity than other compounds.The AChE activity with inhibitory rate of compound 4 was 42.76%,it is higher other compounds,these can be used as basis for further study on endophytic fungus in Uncaria scandens and the development of drugs.

Key words: Uncaria scandens endophytic fungi, secondary metabolites, monoamine oxidase inhibitors, acetylcholinesterase inhibitors