Streptomyces are major producers of active secondary metabolites and show great potential for discovering structurally novel compounds.In-depth analysis of Streptomyces genomic data revealed that a large number of gene clusters producing secondary metabolites were silenced.Therefore,the use of undirected and directed strategies to activate silenced gene clusters in Streptomyces has become a major means of mining structurally novel compounds.In this review,we reviewed the application and progress of non-directed strategies in secondary metabolite mining of Streptomyces,such as changing medium composition or optimizing culture conditions,co-culture,adding chemical elicitors and global regulation,in order to provide reference for the efficient development of secondary metabolites in Streptomyces.