Abstract: In the present study,a simple and efficient preparative procedure was developed for the isolation and purification of water-soluble constituents from Trichosanthes kirilowii Maxim.peel using macroporous absorption resin followed by semi-preparative high performance liquid chromatography (SPHPLC).The water-soluble fraction of T.kirilowii Maxim.peel was firstly chopped up using macroporous absorption resin to yield four subfractions,and they were further purified by SPHPLC,respectively.Finally,nine compounds including three nucleosides and six nucleobases were obtained with high purities,and their structures were confirmed as cytosine,uracil,hypoxanthine,guanine,xanthine,adenine,guanosine,6-isoinosine and adenosine according to UV and NMR analysis.In this work,the chemical constituents of water-soluble fraction of T.kirilowii peel were systematically studied.The developed procedure was simple,effective,scalable and economical, it was a promising alternative procedure for large-scale preparation of polar compounds from natural products.

Key words: Trichosanthes kirilowii Maxim.peel, semi-preparative high performance liquid chromatography, macroporous absorption resin, nucleoside, nucleobase