NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 74-78.doi: 10.16333/j.1001-6880.2015.S.019

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Determination of Icariin in Dahuanglingpi Granule by High Performance Liquid Chromatography

DING Li-yu1, WANG Huan-qing1, WANG Ying-feng2, CHEN Qun-li3, WU Fei2*, XU Bing4*   

  1. 1 Department of Pharmacy,Yueyang Hospital of Integrative Chinese and Western Medicine  Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai,200437,China;2 Shanghai  University of Traditional Chinese Medicine Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine,Ministry of Education,Shanghai,201203,China;3 College of pharmacy Shanghai University of Medicine and Health sciences, Shanghai, 201318,China; 4 Department of Nephrology,Yueyang Hospital of Integrative Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200437,China
  • Online:2015-12-01 Published:2016-01-12

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Abstract: To establish a HPLC method for the determination of icariin in Dahuanglingpi granule.2.0 g sample was ultrasonically extracted for 30 minutes in 25 mL 50% ethanol and determined after filtration.The separation was performed on an Apollo C18 analytical column (4.6×250 mm,5.0 μm) with mobile phase consisting of acetonitrile-water (28:72) at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm and the column temperature was kept at 30 ℃.The linear range of icariin was 0.03836-7.672 μg,the regression equation was Y=1271X+31.68 (r=0.9998) and the recovery at 80%,100% and 120% concentration level was 98.63%,98.95% and 97.86% (RSD was 2.0%,0.5% and 3.2%,respectively, n=3).RSDs of precision,stability and reproducibility tests were less than 1.0%.The developed HPLC method was accurate,simple and suitable for the determination of icariin in Dahuanglingpi granule.

Key words: Dahuanglingpi granule, icariin, high performance liquid chromatography

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