NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 79-83.doi: 10.16333/j.1001-6880.2015.S.020

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Determination of Anthraquinone Derivatives in Rhubarb Seeds

LIU He-chun1,2,3, XU Wen-hua1,2, ZANG Li-li4, ZHOU Guo-ying1,2*   

  1. 1 Northwest Institute of Plateau Biology,Chinese Academy of Sciences; 2 Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences,Xining 810008,China; 3 University of Chinese Academy of Sciences,Beijing 100049,China; 4 University of Electronic Science and Technology of China,Chengdu 610041, China
  • Online:2015-12-01 Published:2016-01-12

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Abstract: The aim of this study was to explore the concentrations of anthraquinone derivatives detected in Rheum tanguticum Maxim.ex Balf.seeds, R.palmatum Linn.seeds and R.officinale Baill.seeds.The contents of aloe-emodin,rhein,emodin,emodin,chrysophanol in rhubarb seeds were determined by highperformance liquid chromatography (HPLC).Agilent Eclipse plus C18 (4.6×250 mm,5 μm) column was used for chromatographic separation with the mobile phase consisting of methanol (A) and 0.1% acetic acid (B) in a gradient elution mode.Column temperature was 25 ℃;Detection wavelength was 254 nm.The results showed that the order of content of concentrations of anthraquinone derivatives was Rh palmatum Linn.seeds > Rheum tanguticum Maxim.ex Balf.seeds > Rh.officinale Baill.seeds.Rhubarb seeds contain abundant emodin,emodin and chrysophanol,excluding aloe-emodin and rhein.The developed method was proved to be accurate,precise,stable and suitable for the determination of anthraquinone derivatives in Rhubarb seeds.

Key words: Rhubarb seeds, HPLC-DAD, anthraquinone derivatives

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