Abstract: Astaxanthin is a kind of natural carotenoids,main extracted from marine animal wastes,algae and red hair,yeast production.In this study,a method was developed for the extraction of astaxanthin from Adonis amurensis. Based on single factor experiments,the effects of extraction solvent,extraction temperature,extraction time and solid-liquid ratio on the extraction yield of astaxanthin were further investigated using orthogonal tests.Experimental results showed that the extraction conditions affected the extraction in the following order:extraction temperature > solid-liquid ratio > extracting time.The optimal extraction conditions were determined as follows:extraction solvent was ethyl acetate,and extraction temperature 40 ℃,solid-liquid ratio of 1:35 (w/w),the extraction time of 3 h.Under the optimized extraction conditions,the extraction yield of astaxanthin reached 81.7%.

Key words: Adonis amurensis, astaxanthin, orthogonal test