NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (5): 696-698.doi: 10.16333/j.1001-6880.2016.5.010

Special Issue: No.4

• Article • Previous Articles     Next Articles

Isolation,Purification and Structural Identification of Component with Antitumor Activity Produced by Streptomyces strain BAF-0711

ZHANG Xin1,YANG Huang-jian2,ZHOU Jian2,CHEN Hong3,FANG Dong-sheng2,HUANG Kai2,ZHENG Wei1,2*   

  1. 1 School of Pharmacy,Fujian Medical University,Fuzhou 350004,China; 2 Fujian Institute of Microbiology, Fuzhou 350007,China; 3 Institute of Materia Medica,Fujian Academy of Traditional Chinese Medicine，Fuzhou 350003,China
  • Online:2016-05-28 Published:2016-09-06

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Abstract: The purpose of the present study was to isolate,purify and identify components with antitumor activity produced by Streptomyces sp. BAF-0711.The secondary metabolite was isolated and purified by macroporous resin column chromatography,silica gel chromatography, etc.;the chemical structure was identified by physicochemical properties and spectroscopic methods;The antitumor activity was detected by MTT assay.As a result,compound BAF-0711-A was isolated from the fermentation broth of Streptomyces sp. BAF-0711 and was identified as bafilomycin A1 by spectroscopic methods.MTT assay showed that BAF0711-A had strong growth inhibitory activity compared with lapatinib on MDA-MB-453 cell,with IC50 value of 0.217 μmol/mL.

Key words: bafilomycin A1, macrolide antibiotic, isolation and purification, structural identification, antitumor activity

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