NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (8): 1233-1237.doi: 10.16333/j.1001-6880.2016.8.011

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Simultaneous Determination of Six Components in Bletilla striata by UPLC-MS/MS

MEI Chao-ye1，4,XIANG Wen-ying1,YANG Wu1,HUANG Yong1,2,WANG Yong-lin1,2,WANG Ai-min1,3*   

  1. 1 Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy;2School of Pharmacy,Guizhou Medical University;3Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education),Guizhou Medical University;4National Engineering Research Center of Miaos Medicines,Guiyang 550004,China
  • Online:2016-08-30 Published:2017-01-17

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Abstract: To establish an UPLC-MS/MS method for the simultaneous determination of α-isobutylmalic acid (B6),blestroside (B12),militarine (B14),gymnosides V (B17),dihydrophenanthrenes 1 (B19) and gymnosides IX (B23) in Bletilla striata. An Acquity UPLC BEH C-18 column（2.1 mm×50 mm，1.7 μm）was used with the mobile phase of 0.1% formic acid in acetonitrile-0.1% formic acid in water by gradient elution.Multiple reaction monitoring (MRM) mode was used for the detection by electro-spray ionization (ESI) source.The results showed that six components can be well resolved and that in the selected linear range,all calibration curves of the six components showed good linearity (r≥0.9974).The average recoveries were 98.07%-103.19%,with RSDs <3.04%. Repeatability,precision and stability were good.The developed method was simple,rapid,sensitive and suitable for the determination of the main active constituents in B.Striata.It provided a new method for the quality control of B.Striata.

Key words: Bletilla striata, UPLC-MS/MS, multiple reaction monitoring, content determination

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