Abstract: In this study,a new strategy for quantitative analysis of multi-components via single marker(QAMS) was developed and validated to analyze four components(geniposide,gallic acid,chlorogenic acid and chebulagic acid) in Sanzisan by HPLC. The QAMS method was based on the relative correction factor(RCF) between each analyte and single standard within certain concentration ranges. In the present study,geniposide was selected as the internal reference substance. The RCFs values between geniposide and the other three components were investigated using three different types of chromatographic columns. The content of other three components were calculated by RCFs,respectively. Meanwhile,the content of the four analytes was also determined by a conventional external standard method to compare with QAMS method. Finally,the relative error which were all under 3% revealed that there was no significant difference between the QAMS method and the external standard method. The QAMS method established in this study solved the problem of the availability and high cost of some standard substances,and would be useful for providing an efficient and feasible quality assessment method for Sanzisan.

Key words: quantitative analysis of multi-components via single marker method, Sanzisan, high performance liquid chromatography