Abstract: This paper presents the saponin constituents from the enzymatic hydrolysate of Panax notoginseng by glucosidase. Eleven saponins were isolated from the hydrolysate of P. notoginseng. On the basis of spectroscopic analysis, their structures were elucidated as  20(S)-protopanaxadiol-20-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (1), ginsenoside compound K (2), 3β,12β,20(S),25-tetrahydroxydammar-23-en-20-O-β-D-glucopyranoside (3), 3β,20(S)-dihydroxydammar-24-en-12β,23β-epoxy-20-O-β-D-glucopyranoside (4), 3β,12β,20(S)-trihydroxy- 25-hydroperoxydammar-23-en-20-O-β-D-glucopyranoside (5), ginsenoside F1 (6), ginsenoside Rg₁ (7), ginsenoside Rg₂ (8), ginsenoside Mc (9), 20(S)-protopanaxadiol-3-O-β-D-xylopyranosyl-(1→2)-β-D- glucopyranosyl-20-O-β-D-glucopyranoside (10) and ginsenoside Re (11), respectively. Among them, compound 1 was a new saponin. In addition, compounds 3-5 and 10  were isolated from P. notoginseng for the first

Key words: Panax notoginseng, ginsenosides, glucosidase