Eight compounds were isolated from the extract of endophytic fungus Talaromyces sp.1.88E from Uncaria rhynchophylla (Miq.) Miq.ex Havil.by various chromatographic techniques.The compounds were identified as (Z)-6-methoxy-6-oxo-2-propylhex-2-enoic acid (1),alteric acid (2),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (3),3-O-methylfunicone (4),alternariol 1′-hydroxy-9-methyl ether (5),alternariol (6),alternariol methyl ether (7),altenuisol (8) by HR-ESI-MS,NMR,IR and other spectroscopic analysis techniques.Among them,compound 1 was a new fatty acid and NMR data of compound 2 was reported for the first time.Compounds 1-8 showed no obvious inhibition of LPS-induced NO production by RAW 264.7 for the anti-inflammatory test.All isolated compounds were evaluated for the inhibitory activities against α-glucosidase and compounds 2,6 showed weak inhibitory activity with IC₅₀ values of 338.5,and 375.8 μmol/L,respectively.