Abstract: In this paper,the antifungal spectrum and its safety to animals of crude antifungal from Bacillus subtilis BS-Z15 fermentation broth was studied.The results showed that,with the exception of Fusarium oxysporum,the crude active substance of BS-Z15 had antagonistic effect on several harmful fungi,such as Verticillium dahliae Kleb,Aspergillus flavus,Candida albican and so on,and the effective concentration of crude substance is 0.10 mg/mL.Its safety was evaluated to the crude antifungal,through acute toxicity oral test on model mices by 32.67 mg/kg lavage.And the results showed,no symptoms of poisoning were detected,death rate of the tested mice was reduced,no significant influence on viscera index,heart and liver tissue characteristics.but it was significant that the activity of antioxidant enzymes was increased,the content of MDA was decreased in liver.In conclusion,the crude antifungal extract from BS-Z15 was safe for mammals and has the potential to be used in the prevention and control of fungal diseases in animals and plants.

Key words: Bacillus subtilis BS-Z15, antifungal active substance, mice, safety