Abstract: It establishedmulti-components with single marker method to simultaneous rapid determining ten kinds of ginsenosides in Shengmai Injection by HPLC,and verified the accuracy.Taking ginsenoside Rb₁ as internal standard substance,it established the relative correction factors among 9 kinds of ginsenosides,and then calculated the content of ginsenosides in the product.And it determined the content of ginsenosides in Shenmai injection by external standard method.Then it proved the feasibility of the determination method with multi-components with single marker through comparing error value between the two methods.The relative correction factors of the ginsenosides were good.The results of comparing between two methods through 10 batches of Shenmai injection determination showed no significant difference.The multi-components with internal standard substance of ginsenosides Rb₁ for 10 kinds of ginsenosides determination in Shenmai injection performed small error.And it could realize the multi compounds quality control of Shenmai Injection.
 

Key words: Shenmai injection, ginsenosides, high performance liquid, multi-components with single marker