NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (12): 2046-2050.doi: 10.16333/j.1001-6880.2019.12.004

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 Chemical constituents of the roots of Millettia speciosa and their inhibitory effects on the formation of osteoclastic cell

LIUYa-lan1,ZHANGYu-ting2,GUQiong2,TENGXi-feng4*,HELin3*   

  1. 1School of Pharmacy,Guangdong Pharmaceutical University;2School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou 510006,China;3School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Guangdong Provincial Cosmetics Engineering and Technology Research Center,Zhongshan 528458，China;4School of Chinese Medicine,Guangdong Pharmaceutical University,State Administration of Traditional
    Chinese Medicine Key Laboratory for Production and Development of Lingnan Medicinal Material,Guangzhou 510006,China
  • Online:2019-12-28 Published:2020-01-10

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Abstract: To investigate the chemical constituents and their inhibitory effects on osteoclastic cell formation activity from the roots of Millettia speciosa,eleven compounds were isolated and identified from the roots of Millettia speciosa through various column chromatography.Based on the NMR and MS spectra,their structures were identified as millettiaosa A(1),maackiain(2),medicarpin(3),lupeol(4),β-sitosterollinoleate(5),β-sitosterol (6),α-monopalmitin(7),(2S)-1-O-heptatriacontanoyl glycerol(8),vanillin(9),methyl hydrogen succinate(10),1-octanol (11).Compounds 5 and 10 were firstly isolated from the genus Millettia.All the isolated compounds 1-11 were evaluated for their inhibitory effects on osteoclastic cell formation.Among of them,compounds 2,3,4 and 6 exhibited potent inhibition with IC50 values of 2.23,1.39,2.25 and 1.63 μM,respectively.Compound 2 was firstly reported inhibitory effects on osteoclastic cell formation activity.

Key words: Millettia speciosa, chemical composition;structural identification;inhibition of osteoclastic cell formation

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