NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (8): 1379-1388.doi: 10.16333/j.1001-6880.2020.8.014

Previous Articles     Next Articles

Optimization of extraction of flavonoids from Prunus cerasifera leaves using response surface methodology and determination of optimum harvest month

ZHEN Ke-miao1,SU Qi1,YAN Zi-han1,GUO Hong-bin1,GAO Jian1,LI Meng-ran2,ZHOU Cheng-yan1*#br#


  1. 1College of Pharmaceutical Science,Key Laboratory of Pharmaceutical Quality Control of Hebei Province,Hebei University,Baoding 071002,China;2College of life sciences,Cangzhou Normal University,Cangzhou 061000,China

  • Online:2020-08-28 Published:2020-09-02


The yield of ultrasonic assisted extraction of cyanidin-3-O-glucoside,rutin,quercetin and kaempferol in the leaves of Prunus cerasifera were determined by high-performance liquid chromatography (HPLC). And according to the activity of the four substances,the weight calculation formula for the yield of flavonoids in the leaves of P. cerasifera was established. On the basis of single-factor test,Box-Behnken response surface methodology was used to optimize extraction process of flavonoids from P. cerasifera leaves. The leaves of P. cerasifera in different months were treated by the best extraction process,and the flavonoids yield was calculated according to the weight of four substances to determine the best picking month. The results showed that the optimum extraction conditions were as follows:ethanol volume fraction was 63.93%,ratio of liquid to raw material was 25∶1 (mL/g),extraction temperature was 44.08 ℃,and extracted for 41.62 min. And the best picking month was August. The results showed good repeatability and high reliability. The yield of cyanidin-3-O-glucoside changed most obviously over time,and was also significantly related to sunshine conditions. All in all,the study in this paper can provide the beneficial reference for the extraction of related components in the future.

Key words: Prunus cerasifera , leaves, flavonoids, response surface methodology, HPLC, ultrasonic assisted extraction

CLC Number: