NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (9): 1582-1591.doi: 10.16333/j.1001-6880.2020.9.017

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Effect of seven different extraction methods on volatile composition of Pandanus amaryllifolius Roxb.

HUANG Yan-li1,2,CHEN Si-ping1,2,GUO Pei-pei2,3,JI Xun-zhi2,4,HAO Chao-yun2,4,YU Huan2,4,QIN Xiao-wei2,4*   

  1. 1College of Tropical Crops,Yunnan Agricultural University,Pu'er 665000,China;2Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning 571533,China;3College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;4Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,Ministry of Agriculture Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning 571533,China

  • Online:2020-09-28 Published:2020-09-25


In order to determine influence of extraction method on volatile composition of Pandanus amaryllifolius Roxb.,100% methanol distillation,75% ethanol distillation,100% ethanol distillation,75% ethanol ultrasonic-assisted extraction,100% ethanol ultrasonic-assisted extraction,75% ethanol extraction,and 100% ethanol extraction have been applied.The relative abundance of volatile compounds from the mature leaves of P. amaryllifolius in Xinglong,Hainan,obtained by different extraction methods,was analyzed by GC-MS.And using principal component analysis and correlation analysis to test the difference of volatile compositions based on the different extraction methods.A total of 52 volatile compositions have been identified in the seven different extraction methods,including esters,alcohols,ketones,olefins and pyrroles.2-Acetyl-1-pyrroline (2-AP) was the main volatile compound of P. amaryllifolius based on the results of six extraction methods except of 100% methanol distillation,and the range of content was 1.21-37.48 μg/g.The correlation analysis showed that the average correlation index was only 34.8%±7.2%.Principal component analysis revealed that the volatile compositions of chlorophyllin,squalene,3-hydroxy-2-butanone,ethyl palmitate,1-hydroxy-2-propanone,3-methyl-2-(5H)-furanone,and 2-AP were strongly correlated with 75%,100% ethanol ultrasonic-assisted extraction and 75%,100% ethanol extraction methods.The volatile compositions of Methyl stearate,2-AP,(E)-7-tetradecanol,and methyl palmitate were correlated with 75%,100% ethanol extraction methods.The volatile compositions of (Z)-hexadecenoic acid methyl ester,styrene,tetradecanoic acid methyl ester,and Pentadecanoic acid methyl ester were correlated with 100% methanol distillation method.These results showed that the content and composition of volatile in P. amaryllifolius was greatly influenced by different extraction method.And the results could be used as a roadmap to guide in the deep processing of pandan products.

Key words: Pandanus amaryllifolius, volatile components, extraction, gas chromatography-mass spectrometry (GC-MS), principal component analysis (PCA), correlation analysis (CA)

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