Protective Effect of Alcohol Extract of Cajanus cajan on Corticosterone-induced Lesion in Cultured PC12 Cells

doi:A

Abstract

Abstract: The aim of this paper was to establish the corticosterone-induced damage model in PC12 cells and study the protective activity of alcohol extracts and its fractions from Cajanus cajan on corticosterone-induced PC12 cells. PC12 cells were exposed to different concentrations of corticosterone to determine the optimum concentration of establishing the damage model, and then the injured PC12 cells were incubated with the alcohol extract and its fractions for 24 h. After that the neuroprotective effect on injured PC12 cells was investigated by morphological observation, LDH detection and MTT determination. The results showed that the survival rate of PC12 cells and the cells exposed to corticosterone (100 μmol/L) for 48 h decreased obviously, with an increase in lactate dehydrogenase (LDH) level, but the effect became weak when the alcohol extracts and its fractions from the C. cajan was added to the PC12 cells, and it was in a dose-dependent manner. The author suggests that the alcohol extract and its fractions from C. cajan can generate a neuroprotective effect on injured PC12 cells induced by corticosterone, and that of alcohol extract is the most significant.

Key words: Cajanus cajan, PC12 cell, conticosterone, MTT, LDH

CLC Number:

R284.2

JIANG Bao-ping, LIU Ya-min, LI Zong-yang, SONG Bo, PAN Rui-le. Protective Effect of Alcohol Extract of Cajanus cajan on Corticosterone-induced Lesion in Cultured PC12 Cells[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1270-.

References

1 Zhou JX(周建新).Reviews on research progresses,actual problem and prospects on natural food preservatives from plat materials.Food Sci(食品科学),2006,27:263-268.
2 Flora of China ,36 volume(中国植物志36卷).Institute of botany,the Chinese Academy of Sciences(中科院植物研究所).Peking:Science press,2006.
3 Zeng XX(曾晓雄),Tan SY(谭淑宜),Luo ZM(罗泽民).Analysis on main chemical components of Malus healthy tea.J Hunan Agric Coll(湖南农学院学报),1993,19:474-476.
4 Deng FX(邓芳席),Huang ZF(黄祖法).Study on bacteriostatic action of Malus melliana leaf.Food Sci(食品科学),1996,7(3):47-50.
5 Xie DP(谢达平),Zao XX(赵肃消),Zeng XX(曾晓雄),et al.Identification of effective mycostatic constituents in Malus meliana leaf.Chem Ind Forest Prod(林产化学与工业),1998,18(2):33-38.
6 Zhong HY(钟海雁),Li ZH(李忠海),Wei YQ(魏元青).The antioxidation and ?OH free radical scavenging of Malus Doumeri leaf extracts.J Beihua University(Natural Science) [北华大学学报(自然科学版)],2001,12:522-525.
7 Wang RC(王融初),Shang BQ(尚本清),Liu ZL(刘宗林).Research and utilization of Malus doumeri leaf.Hunan Forestry Sci Technol(湖南林业科技) 1994,21(3):61-63.
8 Duan XJ,Zhang WW,Li XM,et al.Evaluation of antioxidant property of extract and fractions obtained from a red alga,Polysiphonia urceolata,Food Chem,2006,95:37-43.
9 State Pharmacopoeia Committee of China(国家药典委员会).Pharmacopoeia of the People’s Republic of China,the 2005 version(中华人民共和国药典2005版).Peking:Chemical Industry Press,2005,81.
10 Zhou WJ(周玮婧),Si GH(佀国涵),Sun ZD(孙智达),et al.Antimicrobial activity and mechanism of flavonoids from litchi pericarp.Nat Prod Res Dev(天然产物研究与开发),2011,23:332-336.
11 Salgado H,Lopes C,Lucchesi M.Microbiological assay for gatifloxacin in pharmaceutical formulations.J Pharmaceut Biomed,2006,40:443-446.
12 Stéphanie D,Xavier V,Philippe C,et al.Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH,ABTS,FRAP,SOD and ORAC assays.J Agric Food Chem,2009,57:1768-1774.
13 Xu JG(徐建国),Hu QJ(胡青军).Study on free radical scavenging capacity by cassia seed water extract in vitro.Food Sci(食品科学),2006,27(6):73-76．
14 Thaipong K,Boonprakob U,Crosby K,et al.Comparison of ABTS,DPPH,FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts.J Food Compos Anal,2006,19:669-675.
15 Chow ST,Chao WW,Chung YC.Antioxidative activity and safety of 50% ethanolic red bean extract(Phaseolus radiatus L.var.aurea).J Food Sci,2003,68:21-25.

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