Abstract: One hundred and seven strains of endophytic fungi were isolated from the stem,branch,leaves and bark of root of Chinese yew, T. chinensis of Anhui province.8 endopytic fungi strains which might produce Taxol or its analogues were obtained by preniminary TCL test of the extracts from the fermentation broth of the 107 isolates.The extracts of 8 strains endophytic fungi were further analysed by HPLC,the result showed that HQ-24 strain produced taxol.Screening with CHOcytotoxicity model revealed that HQ-24 strain had relative strong antitumor activity.The extract with antitumor activity from that strain reached the rate of 71.28%.According to morphological characteristics,including hyphae,conidiophores,conidiogenous cells,conidia etc.,HQ-24,the taxol-producing fungus was identified as Penicillium sp.

Key words: endophytic fungi, taxol, tumor cell model, isolated and identified