Abstract: In this study,the α-glucosidase inhibitory activities of petroleum ether fraction of ethanol extract,ethyl acetate fraction of ethanol extract,n-butyl alcohol fraction of ethanol extract,remaining fraction of ethanol extract and aqueous extract of Huidouba were investigated.The influence of shaking during drug enzyme reaction process on the inhibition rate of α-glucosidase was examined by HPLC.The data suggested that Huidouba extracts showed obvious inhibitory effort on α-glucosidase.In addition,during its reaction with α-glucosidase,shaking showed relative large influence on the inhibition rate of α-glucosidase.

Key words: Huidouba, &alpha, -glucosidase, inhibitory activity, HPLC