Optimization of Aqueous Two-Phase Extraction of Saponins from Rubus parvifolius L.by Modified Central Composite Design and Response Surface Methodology

Abstract

Abstract: The aqueous two-phase of ethanol/ammonium sulfate system was used for the extraction of saponins from Rubus parvifolius L..The extraction conditions were optimized using modified central composite design coupled with response surface methodology with two independent variables including quantity of (NH₄)₂SO₄ and CH₃COOH,and the saponin content as the dependent variable.The optimalconditions were determined as follows:12.14% (NH₄)₂SO₄ and 40.00% CH₃COOH. The extraction yield of saponins was 2.95%.The results showed that the central composite design and response surface methodology was useful for optimizing the aqueous two-phase extraction of saponins from R.parvifolius.The developed extraction technology was simple,energy-saving and easy to zoom in industrial production.

Key words: Rubus parvifolius L., saponin, central composite design and response surface methodology, aqueous two-phase system

CLC Number:

R284.2

LI Na1, ZHOU Wen-jie1,2, YU Qi-nan1,2, YANG Rong-ping1,2*. Optimization of Aqueous Two-Phase Extraction of Saponins from Rubus parvifolius L.by Modified Central Composite Design and Response Surface Methodology[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(5): 726-730.

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Optimization of Aqueous Two-Phase Extraction of Saponins from Rubus parvifolius L.by Modified Central Composite Design and Response Surface Methodology

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