Abstract: This study aimed to establish one smiple and fast analytical method for detecting glucosinolate in Maca by high performance liquid chromatography(HPLC).Maca powder was extracted with 80% methanol,then the extractions were absorbed onto a column packed with DEAE-Sephadex A-25,followed by enzymatic hydrolysis with sulfatase,after this step we obtained the sample to be tested.The chromatographic method was carried out on a C18 column using the 0.05% Tetramethylammonium chloride aqueous solution as the mobile phase A and 0.05% Tetramethylammonium chloride aqueous solution(containing 20% acetonitrile,V/V) as B,Benzyl glucosinolate as standard reference material.The flow rate was 1 mL/min on the gradient elution.The monitoring wavelength was at 229 nm.Results showed that linear ranges of glucosinolate was between 0.01790.2684 mg/mL(R²=0.9999).The RSD of precision was 0.052%.The sample recovery rate in different concentrations was 89.2%-93.0%.The detection limit can reach 1 μg/kg.This method is reliable and could be used as a quality control method for glucosinolates.

Key words: maca, glucosinolates, HPLC