Abstract: The leaves of camptotheca acuminate decne are used as the raw material for extraction of camptothecin and 10-hydroxycamptothecin.The leaves are dipped in 0.3% NaOH.Quicklime is added to precipitate the pectin and filtrate it.The liquid is extracted by ethyl alcohol.Then the filtrate liquid is become pH=3 though dropping in hydrochloric acid.The camptothecin and 10-hydroxycamptothecin can be gotten by the 2% and 4% methanol in the chloroform through the silica chromatogram.The rate of yield of camptothecin and 10-hydroxycamptothecin is 0.34‰ and 0.02‰ respectively,and their purity all exceed 98 %(HPLC).

Key words: leaves of camptotheca acuminate decne, Camptothecin, 10-Hydroxycamptothecin, separating