To find the anti-inflammatory substances in Blumea balsamifera oil and study their effects on inflammatory factors of macrophages.In this paper,the anti-inflammatory activities of some compounds in B. balsamifera oil were screened by animal inflammatory model,and then the effects of target compounds on related inflammatory factors in RAW264.7 cells stimulated by LPS were detected.The results indicated that the anti-inflammatory activities of (-)-linalool and trans-caryophyllene were the best in B. balsamifera oil,and different doses of (-)-linalool and trans-caryophyllene could inhibit the production of LTB4,PGE2,NO and iNOS inflammatory mediators and down-regulate the expression of TNF-α,IL-1β,COX-2,5-LOX,FLAP and NF- κB-p65.The results show that (-)-linalool and trans-caryophyllene are important anti-inflammatory substances in B. balsamifera oil,which can exert their anti-inflammatory effects by inhibiting the expression of many kinds of inflammatory mediators,cytokines and NF-κB-p65.