Asperuloside (ASP),an iridoid glycoside,was studied to evaluate its anti-aging effect by detecting the cell viability in human fibroblast BJ cells,followed by a telomeric repeat amplification protocol (TRAP) for analyzing telomerase activity.AutoDock Vina was used to dock asperuloside with 96 selected proteins,to virtually determine the potential anti-aging target,and predict its mechanism.The pretreatment of BJ cell with asperuloside at lower concentrations (0.01 and 0.1 μmol/L) increased viability of 27 population doubling (PD) BJ cells,and it was more active for 34 PD BJ cells.Asperuloside stimulated the telomerase activity in 34 PD BJ cells.Asperuloside had higher than -33.5 kJ/mol binding affinity with 19 proteins including ECH-associated protein 1 (KEAP1),silent information regulator 1 (SIRT1),poly (ADP-ribose) polymerase 1 (PARP1) and so on.Among them,the highest scores of -38.5 and -38.1 kJ/mol were found in docking with KEAP1 and SIRT1.Docked with mammalian target of rapamycin (mTOR),asperuloside also had higner affinity score.These suggested that asperuloside might be a potential stimulating telomerase and anti-aging resource,associated with KEAP1/NRF2,SIRT1,mTOR pathway and so on.