Clerodendrum bungei extract has a good anti-tumor effect.In order to identify the ingredients of the C. bungei extract and explore its impact on the A549 in vitro,the ultra-high-efficiency liquid chromatography-mass spectrometry technology,CCK-8,cell scratch,Transwell,Western blot,immunofluorescence and flow cytometry methods were used to detect. The 34 phenylethanoid glycosides compounds of the C. bungei extract were identified,of which the content of acteoside was 32.95%,and isoacteoside was 5.49%.The C. bungei extract can inhibit the proliferation,migration and invasion of A549 cellsin vitro,and the IC₅₀ is 0.125 mg/mL.In addition,the C. bungei extract can reduce the expression of EphA2,P-AKT,mTOR,P-mTOR,P-GSK-3β,and β-cateninin in A549 cells,increase the expression of EphrinA1,reducing the expression of EphA2 in A549 cell membrane and membrane quality,and blocke the A549 cell cycle to the G1/S stage.This study has confirmed that the C. bungei is rich in phenylethanoid glycosides,especially acteoside and isoacteoside,and can achieve anti-lung cancer effect by regulating the EphA2/AKT/ mTOR pathway.