NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (增刊2): 76-83.

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Study on decolorization technology and antioxidant activity of polysaccharides from non-medicinal parts of Rheum tanguticum

FENG Dan1,2,LONG Ruo-lan1,2,LI Pei-pei1,2,LI Duo1,2,SUN Jing1 *   

  1. 1Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resource,Northwest Institute of Plateau Biology, Chinese Academy of Sciences,Xining 810008,China;2University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2023-12-28 Published:2023-12-11

Abstract:

Rheum tanguticum is a tall perennial herb of Rheum Linn.in Polygonaceae,and its non-medicinal parts have important development and utilization value,a certain amount of polysaccharides are stored in the stems and leaves.Commonly used decolorization methods of polysaccharides include activated carbon method,macroporous resin adsorption method,etc.Graphene has been widely used in recent years because of its strong adsorption capacity.In this paper,three kinds of activated carbon,three kinds of macroporous adsorption resins and amino graphene oxide were selected to discuss the decolorization technology of polysaccharide solution from the non-medicinal parts of R. tanguticum.Then,the decolorization rate and retention rate of polysaccharide under three kinds of decolorizing agents were compared.Meanwhile,the antioxidant capacity of decolorized polysaccharide was determined by DPPH method.The results showed that activated carbon was the best decolorizing agent when only the decolorization rate and retention rate were considered,the decolorization rate and retention rate were 79.59% and 88.72% respectively.The optimum decolorization conditions were as follows:200 mesh activated carbon,pH=5.0,volume ratio of activated carbon∶polysaccharide solution=1∶100,soaking time 20 min,temperature 60 ℃.However,the antioxidant efficiency of the samples treated with activated carbon was the lowest,while the antioxidant activity of the samples treated with graphene and resin was improved.Therefore,in the case of giving priority to antioxidant activity,considering graphene as the best decolorizing agent.As a result,according to the different needs of decolorization,the decolorizing agent and evaluation index should be specifically selected.

Key words: Rheum tanguticum medicinal part, polysaccharise, decolorization process, antioxidant activity

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