This study aims to explore the mechanism of the effect of Cuscuta chinensis flavonoids (CCF) on reducing reproductive toxicity of Tripterygium wilfordii polyside(TWP).A total of 21 four-week-old male rats were randomly divided into normal group,TWP group and CCF group,with seven rats in each group and continuously gavaged for 12 weeks.HE staining was used to observe the histopathological changes of testis and epididymis in rats.The metabolic fingerprints of rat testicular tissues were established by ultra-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and the potential biomarkers of testicular tissue were screened by principal component analysis analysis and orthogonal partial least-squares discrimination analysis in the rat model of reproductive injury intervened by CCF and the Metabo Analyst platform was used for metabolic pathway analysis.The results showed that compared with the TWP group,the spermatogonia at all levels in the CCF group were significantly increased,and the seminiferous tubules were arranged more regularly.A total of 47 differential metabolites such as 1-linolenic glycerophosphocholine,lysophospholipid and phosphatidylethanolamine were obtained by metabolomics technology,which were mainly involved in phenylalanine,tyrosine and tryptophan biosynthesis and metabolism,sphingolipid metabolism and glycerophospholipid metabolism pathways.This study provides a scientific basis for the alleviation of reproductive damage caused by TWP.