Abstract:

This study aims to improve extraction of polyphenols from Sophorae Flavescentis Radix (SFP),which had good ability to lower blood sugar and resist glycation in vitro.The SFP were extracted by ionic liquid-assisted ultrasonic method.The effects of Ionic liquid concentration,extraction time,extraction temperature and ultrasonic power on the extraction yield of SFP were studied by single factor experiment,and the extraction process was optimized by response suriace method based on the resulted of single factor experiment.Meanwhile, α-glucosidase and α-amylase inhibitory activity,and hypoglycemic activity in vitro of SFP was determined.The results showed that the optimal extraction process of SFP was as follows:ionic liquid concentration was 34.6%,material-liquid ratio was 1∶35.4 (g/mL),extraction temperature was 60.6 ℃,extraction time was 29.7 min.The average extraction rate of SFP was 41.27 mg/g by the optimal extraction process,and purified by an AB-8 macroporous resin column,and the purity of SFP was increased from 7.54% to 17.47%.SFP could inhibit the activities of α-glucosidase and α-amylase,and inhibit the glycosylation reaction to reduce blood glucose,respectively,the SFP extracted by ionic liquid-assisted ultrasonic extraction was purified by AB-8 macroporous adsorption resin,which had good ability to lower blood sugar and resist glycation in vitro.

Key words:

polyphenols from Sophorae Flavescentis Radix, extraction and separation, macroporous adsorbent resin, in vitro hypoglycaemia