Abstract:

To elucidate the material basis of the pharmacological effects of leaves of camphor-type Cinnamomum camphora, the study was carried out to isolate and purify the chemical components from the ethyl acetate site of 95% ethanol extract of the plant by Sephadex LH20 gel and silica gel column chromatography and identify the structures of the compounds by modern spectroscopic methods such as NMR, MS, and so on. Furthermore, the inhibitory activities of the above compounds against the mycelial growth of pathogen of anthracnose in C. oleifera were determined by virus-bearing plate method and mycelial growth rate method. Ten compounds were obtained from the ethyl acetate extract of the ethanol extract of the leaves of camphor-type C. camphora, which were narcissoside (1), ayanin (2), bruceine D (3), squalene (4), oleuropein (5), β-carotene (6), vinblastine(7), α-bisabolol (8), isoferulic acid (9) and 7-methylxanthine (10). Among them, compounds 2, 3, 5, 7, 9 and 10 were isolated from leaves of camphor-type C. camphora for the first time. The results of activity screening showed that the inhibition rates of compounds 9, 7 and 2 on the mycelial growth were 62.73%±2.73%, 54.55%±1.82% and 30.91%±5.46% at the concentration of 30 mg/mL.

Key words: Cinnamomum camphora, camphor-type, isolation and identification, chemical compound, antifungal activity