NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (2): 291-301.doi: 10.16333/j.1001-6880.2023.2.013

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Study on the method of simultaneous determination of anti-inflammatory and analgesic alkaloids in Panlongqi tablets

HE Ji1,LIU Yan-ru1 *,DUAN Jin-ao2 *,TANG Zhi-shu1,3,SONG Zhong-xing1,ZHAO Yan-ting1,ZHANG De-zhu4,YANG Guo-wei5   

  1. 1Medicine Shaanxi Provincial and Ministerial Collaborative Innovation Center for Industrialization of Traditional Chinese Medicine Resources,Shaanxi University of Chinese Medicine,Xianyang 712083,China;2 Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China;3China Academy of Chinese Medical Sciences,Beijing 100700，China; 4Shaanxi Panlong Pharmaceutical Group Co.,Ltd.,Shangluo 711400，China;5Shanxi Provincial Inspection and Testing Center (Shanxi Institute of Standards and Metrology Technology),Taiyuan 030002,China
  • Online:2023-02-28 Published:2023-03-02

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Abstract:

The ultra performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technology was utilized to identify the anti-inflammatory and analgesic alkaloids components of Panlongqi tablets,and a total of seventeen alkaloids components were characterized;based on the characterization results,UPLC-ESI-MS/MS quantitative analysis method was established for the quality analysis of Panlongqi tablets.The results showed that seventeen alkaloids had good linear relationship in their respective concentration ranges,r≥0.9990,the average recoveries rate was 86.42%-112.75%,the RSDs of 1.49%-5.59% (n=6).The method established in this study is accurate and reproducible for the quantitative determination of seventeen alkaloids in Panlongqi tablets,which provides a reference for the improvement of potential new indicators for quality control of Panlongqi tablets.

Key words: Panlongqi tablets, alkaloids, composition analysis, UPLC-Q-TOF-MS/MS, UPLC-ESI-MS/MS

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