To investigate the effect of indole-3-carbinol (I3C) on serum metabolites based on ¹H NMR metabolomics in obese mice,twenty-four C57BL/6N mice,consisting of an equal number of males and females,were randomly selected.Based on their body weight,the mice were divided into three groups:normal chow diet group (NCD),high-fat diet group (HFD),high-fat diet and oral intervention of indole-3-carbinol group (HFD-I3C,40 mg/kg).After 12 weeks,serum was collected from the mice.¹H NMR metabolomics technique was used to study the serum metabolites of each group of mice and to obtain the endogenous differential metabolites and related metabolic pathways by multivariate statistical analysis. The results indicated that the differential metabolites identified in comparison across the NCD,HFD,and HFD-I3C groups were argininosuccinic acid and galactose,suggesting that I3C may exert an anti-obesity effect by regulating the metabolism of argininosuccinic acid and galactose.This study′s findings provide a theoretical and experimental basis for understanding the pathogenesis of obesity and the mechanisms by which I3C exerts its anti-obesity effects.